Fig. 3

NAT10 facilitates ac4C modification of CAPRIN1. AÂ NAT10-associated genes were predicted using the LinkedOmics database through Pearson correlation coefficient. Correlation between NAT10 and (B) TRIM44, (C) CAPRIN1, and (D) QSER1 was predicted using the GEPIA database. The expression of (E) TRIM44, (F) CAPRIN1, and (G) QSER1 was measured using qPCR after NAT10 knockdown. HÂ The regulation of NAT10 knockdown on ac4C levels of CAPRIN1 was measured using ac4C-RIP assay. IÂ The relation of NAT10 and CAPRIN1 was evaluated using RIP. JÂ A representative photograph of electrophoresis results in RIP. KÂ Effect of NAT10 on CAPRIN1 mRNA stability was measured using actinomycin D treatment assay. NS: no significance