Fig. 4

Overexpression of CAPRIN1 counteracts the inhibition of OC cellular behaviors caused by NAT10 knockdown. A CAPRIN1 mRNA expression and (B) protein levels in OC cells (CaOV3, A2780, and SKOV3) and normal IOSE-80 cells were examined using qPCR and immunoblotting, respectively. C CAPRIN1 expression in CaOV3 and SKOV3 cells following empty vector and CAPRIN1 overexpressing plasmids transfection. After NAT10 knockdown and CAPRIN1 overexpression, (D) cell migration was evaluated using Transwell assay, and (E) migrated cells were counted; (F) invasion was evaluated using Transwell assay, and (G) invaded cells were counter; (H, I) sphere formation analysis was used to assess stemness; and (J) immunoblotting was conducted to examine the levels of stemness-related factors, including Nanog, OCT4, and SOX2