Fig. 5

Interfering with CAPRIN1 impedes OC cell malignant functions. A After sh-NC and sh-CAPRIN1 transfection, qPCR was performed to measure CAPRIN1 expression in CaOV3 and SKOV3 cells. B After CAPRIN1 knockdown, the protein levels of NAT10 and CAPRIN1 were measured using immunoblotting. C Effect of CAPRIN1 on OC cell migration was assessed using Transwell assay, and (D) the number of migrated cells were counted. E Effect of CAPRIN1 on OC cell invasion was evaluated using Transwell assay, and (F) invasive cell number was counted. G, H Cancer stemness was assessed using sphere formation assay. I The levels of stemness-related proteins (Nanog, OCT4, and SOX2) were measured using immunoblotting