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Fig. 2 | BMC Women's Health

Fig. 2

From: Silencing circRNA-ZFAND6 induces trophoblast apoptosis by activating the mitochondrial pathway through the miR-575/SOD2 axis in unexplained recurrent spontaneous abortion

Fig. 2

Silencing circRNA-ZFAND6 inhibits the proliferation and promotes the apoptosis of HTR8/SVneo cells (A, B) Effects of circRNA-ZFAND6 knockdown or overexpression on cell proliferation were monitored via CCK-8 and EdU assays. Scale bar, 50 μm. (C) The apoptotic rate was detected through flow cytometry transfected with si-circZFAND6 or circZFAND6. (D) The migration ability was assessed by scratch wound healing assay. Magnification, x40. (E) Transwell invasion and migration assay in transfected HTR8/SVneo cells. Scale bar, 100 μm. (F) Effects of circRNA-ZFAND6 silencing or overexpression on the levels of apoptosis-related proteinswere measured via western blot assay. GAPDH served as the internal control. Three different independent experiments with three technical repetitions were performed. Data were expressed as the mean ± SEM. * p<0.05, ** p<0.01, *** p<0.001, ns, not significant

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BMC Women's Health

ISSN: 1472-6874

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